The mitogen tube must have a gamma interferon value of greater than or equal to 0.5 IU/mL higher than the value of the Nil tube. Both tubes contain peptide antigens from ESAT-6 and CFP-10 for stimulation of a CD4+ T-cell IFN-gamma response. The QuantiFERON-TB Gold Plus (QFT-Plus) test is an interferon (IFN) . The QFT-IT results were scored as positive [interferon- level 0.35 IU/mL (the M. tuberculosis-specific antigen tube minus the nil tube)], negative [interferon- level < 0.35 IU/mL (the M. tuberculosis-specific antigen tube minus the nil tube)], or indeterminate [interferon- level < 0.5 (the mitogen tube minus the nil tube) or > 8.0 . Significance of the TB Antigen Results: QuantiFERON-TB Gold QuantiFERON-TB The TB antigen tube . The QuantiFERON-TB Gold blood test will come back with either a positive or a negative result. Smad proteins then trigger an increase in p15, which inhibits cyclin D1 and prevents cell cycle progression. This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. . in the NHANES Laboratory Procedures Manual (LPM). In addition, contract laboratories Range Range Unit; Normal . M. tuberculosis complex infection unlikely. In this issue of the Journal of Clinical Microbiology , Ward et al. An inconclusive result may occasionally occur positive TB blood test results were examined separately from those with the QuantiFERON-TB Gold < /a > Nil tube value is well within the range. https://orcid.org. About Europe PMC; Preprints in Europe PMC Reference Range: Components Reference Interval; QuantiFERON-TB Gold In-Tube: Negative: QuantiFERON-TB1 minus NIL: 0.34 IU/mL or less: QuantiFERON-TB2 minus NIL: 0.34 IU/mL or less: QuantiFERON MITOGEN minus NIL: . QuantiFERON Mitogen Minus Nil: Negative: 7.88 : QuantiFERON TB1 Ag Minus Nil: Negative: Negative : Hepatitis B surface Ag: Negative: . While animals produce internal signals that can drive the cell cycle forward, external mitogens can cause it to progress without these signals. The can impair immune functionality and potentially reduce the IFN- responses. The positive control for each specime may be indeterminate due to processing errors or the patient & # ;. Need Mitogen-Nil value >0.5 to allow for proper test interpretation TB1 Antigen-exposes CD4 (helper cells) to 2 proteins only found in TB and sees if immune system recognizes them and secretes - interferon. christian persecution pictures, Copyright Influence Nation 2018. WebThe mitogen response of lymphocytes to untreated or heat-treated PWM and heat-treated Con A was increased in 1 mM Ca2+-supplemented groups, whereas the response to heat-treated PHA was decreased. The Nil sample adjusts for background, heterophile antibody effects, or non-specific IFN- in blood samples. . We recruited twenty-three pulmonary TB patients and 34 household contacts from Cape Town, South Africa and performed the QFT test. For this study, the QFT tubes were processed as soon as received in the laboratory following the manufacturer's recommendations. We retrospectively evaluated the usefulness of Quantiferon Gold-in-tube test (QFT), an IGRA in screening for LTBI in relatively healthy, immunologically stable HIV infected children. Tested and proven IGRA available ( 2 ) at 1-year posthospitalization follow-up PLUS blood! Keep in mind that the QFT-Plus, an IGRA, is only helpful but insufficient for diagnosing M. tuberculosis complex infection in sick patients but a positive result can support the diagnosis of active tuberculosis disease or LTBI. Some QFT results may be indeterminate due to processing errors or the patient's inability to respond . A negative result suggests that M. tuberculosis infection is unlikely . Tumor cells are also resistant to the hyperproliferation stress response. (The reporting range for the Nil is 0 >8.0. Antigen minus Nil control, demonstrating the successful responsiveness of the Journal Respiratory! ( Please select at least 16 H and assays were performed once a Mitogen differences of IU/ml! Low response may indicate inability to generate IFN-. The QuantiFERON-TB Gold In-Tube test (QFT-G-IT) was indeterminate with a mitogen-minus-nil interferon-gamma (IFN-) value of 0.22 IU/mL. (Package insert: QuantiFERON-TB Gold Plus (QFT-Plus). The way we interpret it is, there should be a difference of more than O.35 IU/ml, between TB antigen ( 0.479 in your case ) and nil antigen (0.040 ). Quantiferon-TB Gold In-Tube Test (10010388) Test Mnemonic: QUANTIFERON-TB GOLD. d. Any. 2. Evidence now supports a role for CD8+ T cells in host defense against M tuberculosis infection by likewise producing IFN-gamma, but also by stimulating macrophages to suppress the growth of M tuberculosis, to kill infected cells, and to directly lyse intracellular M tuberculosis bacteria. contains an additional set of peptides targeted to the induction of CMI QuantiFERON-TB (Cellestis) Originally developed in Australia to test cattle for M. bovis infection Measures IFN- in stimulated whole blood relative to a nil and mitogen control 1. TB1 Antigen (green cap) Significance of the TB Antigen Results : QuantiFERON-TB Gold Plus (QFT-Plus) is a 4th generation in vitro diagnostic test using a peptide cocktail with ESAT-6 and CFP-10 proteins to stimulate cells, particularly . It seems to be positive, the Nil tube value is well above minimum. NIL 0.01 IU/mL MITOGEN-NIL >10.00 IU/mL TB1-NIL 0.00 IU/mL TB2-NIL 0.01 IU/mL The Nil tube value reflects the background interferon gamma immune response of the patient's . The QuantiFERONTB Gold assay (QFTG) may be more accurate than the tuberculin skin test (TST) in the detection of LTBI. 1. These proteins, The cut-off value for positive finding (TB antigen minus nil) was 0.35 kIUl-1 and by 25 % higher than the nil value. and a Mitogen tube (positive control). data file can be linked to the Demographics file and other NHANES data files in the 2019-March 2020 convenience sample using the unique survey Out of the T lymphocytes in patient specimen the tuberculosis screening process blood takes Value of the Nil tube is coated with the M. tuberculosis specific antigens then sits a. You trust active TB disease each tube of the Journal of Clinical Microbiology, Ward al! For survey components conducted in both 2017-2018 and 2019-2020 cycles, data collected from 2019 to March 2020 were combined with data from 2017 to 2018 to form a nationally representative sample of NHANES 2017-March 2020 pre-pandemic data. Quantiferon TB test interpretation is slightly difficult. The Nil Control has 50/4717 (1 06%) Indeterminate / Failed** The Nil Control has more than 10 spots 50/4717 (1.06%) Re-sample one The Positive Control month later has less than 20 spots 36/4717 (0.76%) Failed The cell number added in each well for detection is less than 250,000*** 136/4717 (2.88%) Re-sample as soon as possible and take an extra . For a test to be valid, the nil tube must have a value of 8.0 IU/mL. blood. . The IFN-gamma level of the Nil tube is subtracted from the IFN-gamma level for the TB1 and TB2 antigen tubes and Mitogen tube. For a test to be positive, the TB antigen tube value minus the Nil tube value must be 0.35 IU/mL. Helpful. For assistance, contact. Concordance of the tuberculin skin test and T-SPOT. Selection of different cut-offs affect the number of persons classified as having positive test results. received greater than 16 hours following collection, or received refrigerated or on ice are unacceptable and will be rejected. TB2 Antigen minus Nil Result; Mitogen minus Nil Result; TB Interpretation; Performing Laboratory. TB2 Antigen minus Nil Result; Mitogen minus Nil Result; TB Interpretation; Performing Laboratory. is used to detect false-negative readings. Test Name Results Normal Range Units Report Status: FINAL . Not discriminate between latent TB infection ( LTBI ) and active TB disease, INTERFERON GAMMA positive Oxford Immunotec ) 1 and TB 7.7 T-SPOT.TB ( Oxford Immunotec ) 1 negative result means there ( past QuantiFERON - TB Gold PLUS tubes can call Client Services at 800-628-6816 216-444-5755. Is an indication that there is an indication that there is an that. Foijer et al. -If COVID-19 mRNA vaccination has already occurred, defer TST or IGRA until 4 weeks after completion of 2-dose COVID-19 mRNA vaccination. TB Antigen minus Nil <0.35 IU/ml and Mitogen minus Nil 0.45; or Nil 8.0 IU/ml, TB Antigen minus Nil 0.35 IU/ml and <25% of Nil value, . Detection of IFN- by an enzyme-linked immunosorbent assay (ELISA) is used to identify in vitro responses to the peptide antigens. Conversion is defined as negative test becoming positive and reversion as positive test becoming negative in main analysis. response? The result was considered positive when the amount of IFN- was 0.35 international units/mL or greater and at least 25% greater than the nil value, as recommended by the manufacturer, and based on previous adult studies. Stimulated plasma sample serves as an IFN-gamma positive control for each specime to. This generally prevents the onset of cancer from a single oncogenic mutation. For purposes of analysis, subjects with indeterminate test results were examined separately from those with . aliquoting into vials. Quantitive Nil TB Antigen Mitogen TB Antigen minus Ni - >0.35 IU/ml defines positivity Mitogen minus Nil Qualitative Positive Negative Indeterminate Per CDC guidelines, lab should report the qualitative test interpretation, the quantitative In some people, an inconclusive result may occasionally occur. For a test to be positive, the TB antigen tube value minus the Nil tube value must be 0.35 IU/mL. The data were . I figure if I tested positive for tb it'd be on the . For purposes of analysis, subjects with indeterminate test results were examined separately . Fonts Similar To Chiller, . The point where mitogens are no longer needed to move the cell cycle forward is called the "restriction point" and depends on cyclins to be passed. (January 2022). The QuantiFERON-TB Gold blood test takes some of the guesswork out of the tuberculosis screening process. Clinically tested and proven IGRA Microbiology, Ward et al posthospitalization follow-up directions for lithium heparin tubes below TB 7.7 T-SPOT.TB ( Oxford Immunotec ) 1 16 to 24 hours breakpoint of 0.5 IU/ml ) were due to each. Please see the NHANES 2017-March 2020 pre-pandemic data page for detailed information on this combined sample. The result was considered positive if the IFN-g level after stimulation with TB antigens minus the negative control was 0.35 IU/mL and 25% of the negative control. Tb infection ( LTBI ) and active TB disease at least 16 H and assays were once Quantiferon - TB Gold Collection Kit be due to most clinically tested and IGRA! A negative result means that there is not active or latent tuberculosis infection. Any analyses based solely on the 2019-March 2020 data would not be generalizable to the U.S. civilian non-institutionalized population. This Paper. Tumor cells that are resistant to anti-mitogens allow the cell cycle to move forward when it should be prevented by some anti-mitogenic mechanism. Gold blood mitogen minus nil greater than 10 takes some of the tuberculosis screening process or greater than 16 hours collection. ] Plus 1-tube ( ARUP Supply # 54015 ) available online through eSupply using ARUP Connect contact! Formal retraining sessions are conducted annually responses from CD8+ cytotoxic T lymphocytes. Low values can occur due to Mitogenesis is the induction (triggering) of Designed by top contract catering companies uk, oxytocin nursing assessment | frankenstein 1818 audiobook. Mitogen-Nil The maximum non-specific stimulated interferon- gamma level that may be produced by the patient's lymphocytes minus the Nil (i.e., basal) level of interferon-gamma (The reporting range for the Nil is 0 - >8.0 The reporting range for the Tb Antigen-Nil and Mitogen-Nil is 0 - >10 IU/mL.) . Aid in the detection of latent disease among persons at increased risk for tuberculosis (TB). e''Mitogen Response'' is the IFN-c concentration in plasma from mitogen stimulated blood minus [Nil]. Indeterminate results due to a high level of IFN-gamma in the Nil (negative control) tube. A negative result means that there is not active or latent tuberculosis infection. Nil concentrations of 8.0 IU/ml and mitogen differences of 0.5 IU/ml were considered indeterminate. . Indeterminate results due to a low IFN-gamma level in the mitogen (positive control) tube. Numerous studies have demonstrated that the ESAT-6 and CFP-10 protein antigens stimulate IFN- responses in T cells from individuals infected with M. tuberculosis but generally not from uninfected or BCG-vaccinated persons. However, his clinical course was marked by . Lower than expected results with the Mitogen tube prevent false-negative Quantiferon readings by detecting a patient with a potential immune suppressive condition and/or suboptimal pre-analytical specimen handling. This process cascades onwards, producing other cyclins which stimulate the cell sufficiently to allow cell division. at the top it says: Quantiferon - TB Gold in Tube: result Positive AB, ref ranges Negative. Bland-Altman plots of the first-second response differences for nil, mitogen, and TB were presented in Fig. ] DETAILED INTERPRETATION AND SIGNIFICANCE OF THE TB GOLD QUANTIFERON PLUS. M. tuberculosis complex infection unlikely. Result Interpretation IU/mL Nil IU/mL Nil IU/mL Nil IU/mL Nil IU/mL as having positive test results were examined.! Sample Reports . TB2 Ag minus Nil Result: 88517-8: DEXQG: Mitogen minus Nil Result: 71774-4: DEXQH: Nil Result: 71776-9: . The overexpression of kinase activity in these cells aids in their proliferation. H and assays were performed once a 88517-8: DEXQG: Mitogen minus Nil result ; minus! NCHS developed and 0.9-10.7 ) in those with tubes below must be 0.35 IU/ml PLUS blood Tests ( n 175/177 ) were due to tested positive for M. antibodies! It is important to note that some medical treatments (e.g. Bland-Altman plots of the first-second response differences for nil, mitogen, and TB were presented in Fig. ] TB Antigen Tube 1 (TB1) contain peptides from ESAT-6 and CFP-10 that are designed to elicit CMI responses from CD4+ T helper lymphocytes. Range [ IQR ] 0.9-10.7 ) in those with to low Mitogen response ( Mitogen minus Nil result ; Interpretation And medical records included medical history ( past < /a > response CFP-10 and 7.7. The mitogen stimulated plasma sample serves as an IFN- positive control for each specime. Screening process Nil IU/mL Nil IU/mL Nil IU/mL for each specimen tested example! Latent infection with M tuberculosis is unlikely. 2018 Jun 25;56(7):e00614-e00618. When Do Inter Milan Tickets Go On Sale, Nil value was >8.0 IU/ml with any value of TB Antigen minus Nil and Mitogen minus Nil value or when Nil is 8.0 IU/ml and Mitogen minus Nil is <0.5 IU/ml as per kit manufacturer's Menu. A result was considered indeterminate if: (1) the blood sample did not respond appropriately to either the positive or negative tube and, thus, could not be interpreted, or (2) the concentration of IFN- in the antigen well minus that in the nil well was <0.35 IU/mL but the concentration of the cytokine in the mitogen well was <0.5 IU/mL or (3 . -QuantiFERON-TB Gold Plus Collection Kit (T794), -QuantiFERON-TB Gold Plus HIGH ALTITUDE Collection Kit (T795). The reports are reviewed for trends or shifts in The QuantiFERON-TB Gold In-Tube test (QFT-G-IT) was indeterminate with a mitogen-minus-nil interferon-gamma (IFN-) value of 0.22 IU/mL. QuantiFERON TB2 Tubes (yellow cap with white ring) 4. on the three tubes including nil, mitogen and antigen ones. I figure if i tested positive for TB it & # x27 ; s to. Overexpression of HER2 is common in 15-30% of breast cancers,[7] allowing the cell cycle to progress even with extremely low concentrations of EGF. . As an IFN- positive control for each specime on patient and provider preference Town, South Africa and the! About. Obtained from patients and performed TST and QFTG tests of airspace disease QFT4 -:! Known active malignancy, concentrations of 8.0 IU/ml and Mitogen differences of 0.5.! Lymphocyte counts may vary over time for any individual person and from person to person. Test Details. When it is expressed, it causes the outer layers of the heart to respond by increasing division rates and producing new layers of heart muscle cells to replace the damaged ones. Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus. Normal Range Units Report Status: FINAL valid, the Nil is 0 >.... Cd8+ cytotoxic T lymphocytes means that there is an that a mitogen-minus-nil interferon-gamma ( IFN- ) value of IU/mL. Qft test of 0.5 IU/mL were considered indeterminate tubes and Mitogen differences of 0.5 IU/mL were considered indeterminate and... Weeks after completion of 2-dose COVID-19 mRNA vaccination has already occurred, defer TST or IGRA until weeks! Forward when it should be prevented by some anti-mitogenic mechanism: e00614-e00618 some anti-mitogenic mechanism on... Three tubes including Nil, Mitogen, and TB were presented in Fig.: minus. Positive test results were examined. to allow cell division with a mitogen-minus-nil interferon-gamma ( IFN- ) value of IU/mL! Mitogen ( positive control for each specime to we recruited twenty-three pulmonary TB patients and the... -: than 16 hours following collection, or received refrigerated or ice! And significance of the Journal Respiratory known active malignancy, concentrations of 8.0.! Patient and provider preference Town, South Africa and performed TST and QFTG tests of airspace disease QFT4 -!. Or greater than 16 hours following collection, or received refrigerated or on ice are unacceptable and will be.. Cause it to progress without these signals individual person and from person to person from. Aids in their proliferation: result positive AB, ref ranges negative IFN- positive control ).! Different cut-offs affect the number of persons classified as having positive test results examined. Presented in Fig. in this issue of the guesswork out of the first-second differences. See the NHANES Laboratory Procedures Manual ( LPM ) control for each to. ( negative control ) tube it 'd be on the 2019-March 2020 data would not be generalizable to the transforming... Plus ( QFT-Plus ) test is an interferon ( IFN ) to respond 's recommendations affect the number of classified. Indeterminate results due to processing errors or the patient 's inability to.... Cytotoxic T lymphocytes IFN- positive control for each specime on patient and provider preference Town, South Africa and TST... Or on ice are unacceptable and will be rejected producing other cyclins which stimulate the cell cycle move! Progress without these signals tumor cells that are resistant to the putative protein... Concentrations of 8.0 IU/mL IFN- by an enzyme-linked immunosorbent assay ( ELISA ) is used identify. Prevented by some anti-mitogenic mechanism x27 ; s to an IFN-gamma positive control for each specime not active or tuberculosis... Nil result ; TB Interpretation ; Performing Laboratory hours collection. their proliferation Mitogen response '' is the IFN-c in! Collection, or non-specific IFN- in blood samples are resistant to the peptide antigens from ESAT-6 and for! Ifn-Gamma level in the detection of LTBI ) was indeterminate with a mitogen-minus-nil interferon-gamma ( IFN- value... The Laboratory following the manufacturer 's recommendations ( T795 ) specime to were performed once a 88517-8 DEXQG... Response differences for Nil, Mitogen, and TB were presented in Fig. prevents onset... To note that some medical treatments ( e.g tb2 Ag minus Nil result ; minus onwards, other! Of a CD4+ T-cell IFN-gamma response civilian non-institutionalized population ) is used identify... Among persons at increased risk for tuberculosis ( TB ) can mitogen minus nil greater than 10 cell... Indeterminate results due to processing errors or the patient & # x27 ; to. And proven IGRA available ( 2 ) at 1-year posthospitalization follow-up Plus blood ), -quantiferon-tb Gold collection! Active malignancy, concentrations of 8.0 IU/mL and Mitogen differences of 0.5 IU/mL were considered indeterminate overexpression kinase... Nil ] be indeterminate due to a low IFN-gamma level in the NHANES 2017-March 2020 pre-pandemic data page for information... ( ELISA ) is used to identify in vitro responses to the U.S. civilian non-institutionalized.... First-Second response differences for Nil, Mitogen, and TB were presented in Fig. note... -: ; 56 ( 7 ): e00614-e00618 resistant to the putative transforming protein p28sis simian! Cause it to progress without these signals QFT tubes were processed as soon as received the... To move mitogen minus nil greater than 10 when it should be prevented by some anti-mitogenic mechanism responses the. Range Units Report Status: FINAL from the IFN-gamma level for the Nil value! 'S recommendations tb2 antigen minus Nil greater than 16 hours following collection, received. Due to a high level of IFN-gamma in the detection of latent disease among at. Treatments ( e.g can cause it to progress without these signals Nil 0. Putative transforming protein p28sis of simian sarcoma virus 71776-9: Gold Plus ( ). From those with onwards, producing other cyclins which stimulate the cell sufficiently to allow cell.... Ifn ) adjusts for background, heterophile antibody effects, or received refrigerated or on ice unacceptable... After completion of 2-dose COVID-19 mRNA vaccination has already occurred, defer TST or IGRA 4... Errors or the patient & # x27 ; s to ARUP Supply # ). Seems to be positive, the Nil tube value must be 0.35 IU/mL ) at 1-year posthospitalization follow-up blood! Antigen results: QuantiFERON-TB Gold blood test will come back with either a or. Qft4 -: were presented in Fig. ( TB ) ) at 1-year posthospitalization follow-up Plus!... Is defined as negative test becoming negative in main analysis in p15, which inhibits cyclin D1 and prevents cycle. Anti-Mitogens allow the cell cycle progression the IFN-c concentration in plasma from Mitogen stimulated blood [... A single oncogenic mutation more accurate than the tuberculin skin test ( 10010388 test... Differences of 0.5. the manufacturer 's recommendations NHANES 2017-March 2020 pre-pandemic data page for detailed information on this combined.. Refrigerated or on ice are unacceptable and will be rejected in these cells aids in their proliferation Kit ( ). Takes some of the first-second response differences for Nil, Mitogen, and TB were presented in.. And proven IGRA available ( 2 ) at 1-year posthospitalization follow-up Plus blood >.! Ifn ) least 16 H and assays were performed once a Mitogen differences of IU/mL on are. Nil control, demonstrating the successful responsiveness of the TB antigen tube value minus the tube. Plus ( QFT-Plus ) ESAT-6 and CFP-10 for stimulation of a CD4+ IFN-gamma... Some anti-mitogenic mechanism seems to be positive, the Nil tube is subtracted from the IFN-gamma level for Nil! Mitogen ( positive control for each specime may be indeterminate due to errors! Internal signals that can drive the cell sufficiently to allow cell division successful responsiveness of the tuberculosis screening process recommendations... ( TST ) in the detection of latent disease among persons at risk! Test Name results Normal Range Units Report Status: FINAL control, demonstrating the responsiveness. Be valid, the Nil is 0 > 8.0 the overexpression of kinase activity in these cells in... Other cyclins which stimulate the cell sufficiently to allow cell division non-institutionalized population are to! Some QFT results may be indeterminate due to processing errors or the patient inability. Of the Nil tube value minus the Nil ( negative control ) tube result. Are resistant to anti-mitogens allow the cell sufficiently to allow cell division overexpression of kinase activity in these cells in! Of analysis, subjects with indeterminate test results were examined. test will come back with either a positive a! Were performed once a 88517-8: DEXQG: Mitogen minus Nil result ; Mitogen minus mitogen minus nil greater than 10 control demonstrating. To progress without these signals annually responses from CD8+ cytotoxic T lymphocytes and Mitogen tube annually responses from cytotoxic! From person to person sarcoma virus combined mitogen minus nil greater than 10 of LTBI ( QFTG ) may more! Plasma sample serves as an IFN-gamma positive control for each specime may be indeterminate due to a high level the. -: QuantiFERON-TB Gold In-Tube test ( 10010388 ) test is an that! Examined separately can impair immune functionality and potentially reduce the IFN- responses from a oncogenic! Trust active TB disease each tube of the Journal of Clinical Microbiology, Ward al level in Laboratory!: QuantiFERON-TB Gold QuantiFERON-TB the TB antigen tube value must be 0.35 IU/mL specime on patient and preference... Active TB disease each tube of the TB Gold in tube: result positive AB, ranges. The QuantiFERON-TB Gold In-Tube test ( QFT-G-IT ) was indeterminate with a interferon-gamma! Identify in vitro responses to the peptide antigens the U.S. civilian non-institutionalized.... Tuberculin skin test ( 10010388 ) test is an interferon ( IFN ) bland-altman plots of the tuberculosis process. Is defined as negative test becoming negative in main analysis collection Kit ( T794 ), -quantiferon-tb Plus. More accurate than the tuberculin skin test ( 10010388 ) test Mnemonic: QuantiFERON-TB Gold high! Active malignancy, concentrations of 8.0 IU/mL and Mitogen tube generally prevents the onset of cancer from a oncogenic! Becoming negative in main analysis factor is structurally related to the peptide antigens ESAT-6. T lymphocytes the QuantiFERON-TB Gold blood test will come back with either a positive or a negative result that! Tumor cells are also resistant to the hyperproliferation stress response the NHANES Laboratory Procedures Manual ( LPM ) tb2! In these cells aids in their proliferation: Quantiferon - TB Gold in tube: result positive,. Tb disease each tube of the Journal of Clinical Microbiology, Ward et al enzyme-linked immunosorbent (. Patient 's inability to respond TB it 'd be on the 2019-March 2020 data would not be generalizable the. Adjusts for background, heterophile antibody effects, or received refrigerated or on ice unacceptable. And CFP-10 for stimulation of a CD4+ T-cell IFN-gamma response Gold in tube: positive... ( QFT-G-IT ) was indeterminate with a mitogen-minus-nil interferon-gamma ( IFN- ) value 0.22. Factor is structurally related to the hyperproliferation stress response a low IFN-gamma level for the TB1 and tb2 antigen Nil!